roche lightcycler 96 (Roche)
Roche manufactures this product 
99
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Roche
roche lightcycler 96
Roche Lightcycler 96, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/roche+lightcycler+96/LightCycler+480+System/pmc12874286-701-8-8
Average 99 stars, based on 6 article reviews
Roche Lightcycler 96, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/roche+lightcycler+96/LightCycler+480+System/pmc12874286-701-8-8
Average 99 stars, based on 6 article reviews
roche lightcycler 96 - by Bioz Stars,
2026-09
99/100 stars
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Amplification:Article Title: ALKBH5 facilitates Srgn stability via m6A demethylation to disrupt blood-testis barrier integrity in the high-fat diet mice. Article Snippet: Total RNA was extracted using a Total RNA Isolation Kit (CWBIO, CW0581S) and reverse-transcribed with Evo M-MLV RT Mix Tracking Kit (Accurate Biology, AG11734). .. Then, transcripts were amplified using AceQ® qPCR SYBR Green Master Mix (Vazyme, Q121) by Real-time Polymerase Chain Reaction:Article Title: ALKBH5 facilitates Srgn stability via m6A demethylation to disrupt blood-testis barrier integrity in the high-fat diet mice. Article Snippet: Total RNA was extracted using a Total RNA Isolation Kit (CWBIO, CW0581S) and reverse-transcribed with Evo M-MLV RT Mix Tracking Kit (Accurate Biology, AG11734). .. Then, transcripts were amplified using AceQ® qPCR SYBR Green Master Mix (Vazyme, Q121) by Article Title: Integrated cryopreservation-thawing-transplantation platform for neural stem cell-based spinal cord injury repair Article Snippet: Subsequently, RT-qPCR assays were performed with UltraSYBR Mixture (CWBio, China). .. All PCR reactions were carried out on a Article Title: Low-frequency electromagnetic fields ameliorate testosterone-induced androgenetic alopecia in mice through LncRNA H19/miR-214-5p/β-catenin signal pathway Article Snippet: Images were captured using an Olympus IX61 FL microscope and analyzed with Image-Pro Plus. .. The cDNA for lncRNAs, mRNAs, and miRNAs was synthesized using the PrimeScriptTM RT kit (Takara, Dalian, China), with stem-loop primers for miRNAs and U6 as control. qPCR was performed with TB GreenTM Premix Ex TaqTM II on a Article Title: Origin and evolution of BZR gene family in plants, pan-genome analysis of the BZR1 gene family, and functional characterization of CaBZR1.2 in pepper lateral branch development Article Snippet: Total RNA was isolated using a plant RNA extraction kit (Cwbio, Taizhou, China). cDNA was synthesized from 1 μg of total RNA using a reverse transcription kit (Vazyme, Nanjing, China). .. RT-qPCR analysis was performed using SYBR Green (Vazyme, Nanjing, China) on a Article Title: Identification of Candidate Heat-Tolerance Genes in Maize by Integrating Linkage and Transcriptomic Analyses. Article Snippet: .. The qRTPCR reactions were carried out on a Article Title: Neuroprotective effects of hydrogen sulfide on BDNF, IGF-1, and oxidative stress in the hippocampus of high-fat diet rats Article Snippet: Only high-quality RNA samples were used for cDNA synthesis and qPCR analyses, which was performed with a commercial cDNA synthesis kit (Yekta Tajhiz Azma, Iran). .. Relative gene expression was quantified using the Article Title: Enhanced anticancer activity of nanoemulsified cardamom extract via modulation of apoptosis- and lncRNA-associated pathways in colorectal cancer cells Article Snippet: .. Quantitative polymerase chain reaction (qPCR) was performed in a volume of 20 μl containing 10 μl SYBR Green (2X, Pars Toos, Mashhad, Iran), 0.5 μl each of forward and reverse primers, and 20 ng of template cDNA in a SYBR Green Assay:Article Title: ALKBH5 facilitates Srgn stability via m6A demethylation to disrupt blood-testis barrier integrity in the high-fat diet mice. Article Snippet: Total RNA was extracted using a Total RNA Isolation Kit (CWBIO, CW0581S) and reverse-transcribed with Evo M-MLV RT Mix Tracking Kit (Accurate Biology, AG11734). .. Then, transcripts were amplified using AceQ® qPCR SYBR Green Master Mix (Vazyme, Q121) by Article Title: Origin and evolution of BZR gene family in plants, pan-genome analysis of the BZR1 gene family, and functional characterization of CaBZR1.2 in pepper lateral branch development Article Snippet: Total RNA was isolated using a plant RNA extraction kit (Cwbio, Taizhou, China). cDNA was synthesized from 1 μg of total RNA using a reverse transcription kit (Vazyme, Nanjing, China). .. RT-qPCR analysis was performed using SYBR Green (Vazyme, Nanjing, China) on a Article Title: Enhanced anticancer activity of nanoemulsified cardamom extract via modulation of apoptosis- and lncRNA-associated pathways in colorectal cancer cells Article Snippet: .. Quantitative polymerase chain reaction (qPCR) was performed in a volume of 20 μl containing 10 μl SYBR Green (2X, Pars Toos, Mashhad, Iran), 0.5 μl each of forward and reverse primers, and 20 ng of template cDNA in a Polymerase Chain Reaction:Article Title: Integrated cryopreservation-thawing-transplantation platform for neural stem cell-based spinal cord injury repair Article Snippet: Subsequently, RT-qPCR assays were performed with UltraSYBR Mixture (CWBio, China). .. All PCR reactions were carried out on a Article Title: Apicidin compromises oocyte quality by disrupting meiotic apparatus and histone acetylation. Article Snippet: Apicidin, an emerging mycotoxin increasingly detected in cereal crops and animal feed, has been reported to exert adverse effects on animals and cell lines.. Oocyte maturation is essential for successful fertilization and early embryonic development.. However, the effects of Apicidin (AP) exposure on oocyte maturation remain unclear. Synthesized:Article Title: Low-frequency electromagnetic fields ameliorate testosterone-induced androgenetic alopecia in mice through LncRNA H19/miR-214-5p/β-catenin signal pathway Article Snippet: Images were captured using an Olympus IX61 FL microscope and analyzed with Image-Pro Plus. .. The cDNA for lncRNAs, mRNAs, and miRNAs was synthesized using the PrimeScriptTM RT kit (Takara, Dalian, China), with stem-loop primers for miRNAs and U6 as control. qPCR was performed with TB GreenTM Premix Ex TaqTM II on a Control:Article Title: Low-frequency electromagnetic fields ameliorate testosterone-induced androgenetic alopecia in mice through LncRNA H19/miR-214-5p/β-catenin signal pathway Article Snippet: Images were captured using an Olympus IX61 FL microscope and analyzed with Image-Pro Plus. .. The cDNA for lncRNAs, mRNAs, and miRNAs was synthesized using the PrimeScriptTM RT kit (Takara, Dalian, China), with stem-loop primers for miRNAs and U6 as control. qPCR was performed with TB GreenTM Premix Ex TaqTM II on a Quantitative RT-PCR:Article Title: Origin and evolution of BZR gene family in plants, pan-genome analysis of the BZR1 gene family, and functional characterization of CaBZR1.2 in pepper lateral branch development Article Snippet: Total RNA was isolated using a plant RNA extraction kit (Cwbio, Taizhou, China). cDNA was synthesized from 1 μg of total RNA using a reverse transcription kit (Vazyme, Nanjing, China). .. RT-qPCR analysis was performed using SYBR Green (Vazyme, Nanjing, China) on a Article Title: Apicidin compromises oocyte quality by disrupting meiotic apparatus and histone acetylation. Article Snippet: Apicidin, an emerging mycotoxin increasingly detected in cereal crops and animal feed, has been reported to exert adverse effects on animals and cell lines.. Oocyte maturation is essential for successful fertilization and early embryonic development.. However, the effects of Apicidin (AP) exposure on oocyte maturation remain unclear. Gene Expression:Article Title: Neuroprotective effects of hydrogen sulfide on BDNF, IGF-1, and oxidative stress in the hippocampus of high-fat diet rats Article Snippet: Only high-quality RNA samples were used for cDNA synthesis and qPCR analyses, which was performed with a commercial cDNA synthesis kit (Yekta Tajhiz Azma, Iran). .. Relative gene expression was quantified using the |